Coding
eGFP+TmTHP

Part:BBa_K193203:Design

Designed by: Nao Nakatani   Group: iGEM09_Tokyo_Tech   (2009-10-14)


TmTHP with EGFP for fusion of Antifreeze protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 993
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 993
    Illegal NheI site found at 1011
    Illegal NotI site found at 1023
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 993
    Illegal BamHI site found at 528
    Illegal XhoI site found at 429
    Illegal XhoI site found at 1032
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 993
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 993
    Illegal AgeI site found at 738
    Illegal AgeI site found at 777
    Illegal AgeI site found at 849
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The gene sequence was obtained from clonetech.

References